{"title":"Serum Free and Protein free","description":"","products":[{"product_id":"protein-free-medium-pf486a","title":"Protein-Free CHO Medium (PF486A)","description":"\u003ctable style=\"border-collapse: collapse;\" width=\"100%\" data-mce-style=\"border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid black; width: 46.6089%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 46.6089%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/PF_CHO_Medium_PF486A.pdf?v=1682330953\" target=\"_blank\"\u003e\u003cstrong\u003e제품 설명서 (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid black; width: 53.3911%; text-align: center;\" colspan=\"2\" data-mce-style=\"border: 1px solid black; width: 53.3911%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/Protein-Free_CHO_Medium_PF486A_GHS_MSDS.pdf?v=1682337641\" target=\"_blank\" rel=\"noopener noreferrer\"\u003e\u003cstrong\u003eMSDS (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProtein-Free CHO Medium, Liquid\u003cbr\u003e\u003c\/strong\u003eWith 4500 mg\/L D-glucose\u003cbr\u003eWith HEPES\u003cbr\u003eWith Pluronic® F68\u003cbr\u003eWith 4 mM L-glutamine\u003cbr\u003eWith sodium bicarbonate\u003cbr\u003eFor the suspension culture of CHO cell\u003c\/p\u003e\n\u003cp\u003eCatalog Number \u003cstrong\u003ePF 486A\u003cbr\u003e\u003c\/strong\u003eStorage Temperature 2~8°C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003cbr\u003e\u003c\/strong\u003eProtein-Free CHO Media were developed to support the cell growth of Chinese Hamster Ovary cells in suspension cultures. Protein-Free CHO Medium differs from normal (serum supplemented) media in its amino acid, vitamin, and mineral composition, and includes peptides, fatty acids, and growth factors as substitutes to serum. When transplanting CHO cells that had been formerly cultured in a serum supplemented medium, direct adaptation or sequential adaptation should be administered for the appropriate amount of time. When trypsin is used in sub-cultivation of cells in an adherent culture, trypsin inhibitor is supplemented in place for serum for neutralizing effects. A centrifuge process followed by cell cleansing eliminates trypsin from the formulation. The absence of trypsin allows culture media to be transferred into a new container and continue to sustain cell growth with added Protein-Free Medium. If needed, the medium is to be centrifuged at 800 rpm for 5 minutes prior to transfer.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePF 486A \u003c\/strong\u003econtains 4500 mg\/L D-glucose, 4 mM L-glutamine, sodium bicarbonate, and HEPES. Pluronic® F-68 is supplemented to prevent damage to CHO cells in a suspension culture. Hypoxanthine and thymidine should be supplemented separately when required for cell growth. \u003cstrong\u003ePF 486A\u003c\/strong\u003e vary in their compositions and consequently yield different effects on different cell lines. The optimal product for a certain cell type can only be confirmed through direct application. \u003cstrong\u003ePF 486A\u003c\/strong\u003e is produced free of materials of animal origin, thus making them appropriate for the research and production of medical quality recombinant proteins. In selecting the appropriate culture environment of Protein-Free Medium, (1) the origin of the cell host (the kind of cell), (2) the type of culture container to be used (tissue flask, multiplayer flasks, roller bottle, spinner flasks, or bioreactors), and (3) the characteristics of the CHO cell line is important factors to be considered.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eStorage\/Stability\u003cbr\u003e\u003c\/strong\u003eThe Protein-Free medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and\/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eInstructions for Use\u003cbr\u003e\u003c\/strong\u003e(1) \u003cstrong\u003ePF 486A\u003c\/strong\u003e is generally supplemented with 2~8 mM of L-glutamine (\u003cstrong\u003eLS 002-01\u003c\/strong\u003e), (2) nutritional requirements can be altered according to the gene expression system, (3) the concentration of D-glucose (\u003cstrong\u003eLS 001-01\u003c\/strong\u003e or \u003cstrong\u003eLS 001-02\u003c\/strong\u003e) can be adjusted up to 6.8 g\/L, (4) polyamine supplement (\u003cstrong\u003eLS 018-01\u003c\/strong\u003e), fatty acid supplement (\u003cstrong\u003eLS 017-01\u003c\/strong\u003e), sodium butyrate (\u003cstrong\u003eLS 033-01\u003c\/strong\u003e) is recommended for encouraging the expression of recombinant proteins, (5) when cultivating in a CO\u003csub\u003e2\u003c\/sub\u003e incubator, adjustment of pH should be made by altering NaHCO\u003csub\u003e3 \u003c\/sub\u003econcentration, (6) when preparing a suspension culture in a spinner flask, allow 1\/4 of the tray to be open to ensure aeration.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBiological Performance Characteristics\u003cbr\u003e\u003c\/strong\u003eThe growth-promoting capacities of the Protein-Free CHO media are tested in a liquid medium using CHO cell. Growth rates are examined through three subculture. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePrecautions\u003cbr\u003e\u003c\/strong\u003eProtein-Free CHO media are only used \u003cem\u003eIn Vitro, \u003c\/em\u003eand are sensitive to light and heat. Adhering to the following suggestions can increase the product shelf life. (1) Limit light exposure, (2) avoid keeping at room temperature (around 37°C), (3) store in a darkroom at 2~8°C, (4) use in small amounts at a time.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAdaptation of CHO cells to Protein-Free media\u003cbr\u003e\u003c\/strong\u003eA sequential adaptation protocol may be necessary if direct adaptation does not work.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003cstrong\u003e Direct Adaptation (1)\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eIf CHO cell confluence reaches more than 80% in serum supplemented medium, remove culture medium.\u003c\/li\u003e\n\u003cli\u003eCell, remove using Trypsin or Trypsin-EDTA for 2~3 minutes at 37°C and cell neutralizes using serum supplemented medium. And then remove supernatant by centrifugation cells at 800 rpm for 5 minutes.\u003c\/li\u003e\n\u003cli\u003eCell suspended using Protein-Free medium (contained L-glutamine). And remove supernatant by centrifugation cells at 800 rpm for 5 minutes.\u003c\/li\u003e\n\u003cli\u003eResuspend cells to a viable cell density of 2~5X10\u003csup\u003e5\u003c\/sup\u003e cells\/mL in Protein-Free medium. Incubate the cells in spinner flask at 40 rpm.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003cstrong\u003e Direct Adaptation (2)\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eIf CHO cell confluence reaches more than 90% serum supplemented medium, convert culture medium into Protein-Free medium.\u003c\/li\u003e\n\u003cli\u003eGather and culture suspension cell in T-flask of one while subculture the cells using Protein-Free medium.\u003c\/li\u003e\n\u003cli\u003eConfirmed growth cell is subculture. Resuspend cells to a viable cell density of 2~5X10\u003csup\u003e5\u003c\/sup\u003e cells\/mL in Protein-Free medium. Incubate the cells in spinner flask at 40 rpm.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003cstrong\u003e Sequential Adaptation\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eIf CHO cell confluence reaches more than 80% serum supplemented medium, remove cell using Trypsin or Trypsin-EDTA. Incubate cells at viable cell density of 3~4X10\u003csup\u003e5\u003c\/sup\u003e cells\/mL in a 75:25 (v\/v) mixture of serum supplemented:Protein-free medium.\u003c\/li\u003e\n\u003cli\u003eRepeat subculture 2~4 times in same conditions and monitor the culture until the density reaches 90 % viable cells\/mL compare with existing culture. Then subculture at viable cell density of 3~4X10\u003csup\u003e5\u003c\/sup\u003e cells\/mL into a 50:50 (v\/v) mixture of serum supplemented:Protein-Free medium.\u003c\/li\u003e\n\u003cli\u003eRepeat process 2, do subculture into a 25:75 (v\/v) mixture of serum supplemented:Protein-Free medium.\u003c\/li\u003e\n\u003cli\u003eRepeat process 2, do subculture into a Protein-Free medium only.\u003c\/li\u003e\n\u003cli\u003eIf cell growth is confirmed, cell freeze using freezing medium (contained Protein-Free medium) and examines cell activity after thawing.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Welgene","offers":[{"title":"1L","offer_id":43540207501529,"sku":null,"price":108000.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/products\/PF486A.jpg?v=1671495164"},{"product_id":"serum-free-hybridoma-medium-sf401","title":"Serum-Free Hybridoma Medium  (SF401)","description":"\u003ctable width=\"100%\" style=\"border-collapse: collapse;\" data-mce-style=\"border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid black; width: 46.6089%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 46.6089%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/SF_Hybridoma_Medium_SF401.pdf?v=1681950110\" target=\"_blank\"\u003e\u003cstrong\u003e제품 설명서 (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd colspan=\"2\" style=\"border: 1px solid black; width: 53.3911%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 53.3911%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/Serum-Free_Hybridoma_Medium_SF401_GHS_MSDS.pdf?v=1682337641\" target=\"_blank\" rel=\"noopener noreferrer\"\u003e\u003cstrong\u003eMSDS (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSerum-Free Hybridoma Medium, Liquid\u003cbr\u003e\u003c\/strong\u003eWith D-glucose\u003cbr\u003eWith sodium bicarbonate\u003cbr\u003eWith HEPES\u003cbr\u003eWith L-glutamine\u003cbr\u003eWith Pluronic® F68\u003cbr\u003eFor the adherent culture of Hybridoma cell\u003c\/p\u003e\n\u003cp\u003eCatalog Number \u003cstrong\u003eSF 401\u003cbr\u003e\u003c\/strong\u003eStorage Temperature 2~8°C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003cbr\u003e\u003c\/strong\u003eSerum-Free Hybridoma Media are liquid media formulated to support suspension culture of hybridoma cells. Serum-Free Hybridoma Media formulations include the minimal amount of protein required for cell growth. Serum-Free Hybridoma Media differs from normal (serum supplemented) media in its amino acid, vitamin, and mineral composition, and includes peptides, fatty acids, and growth factors as substitutes to serum. Hybridoma cells that had been formerly cultured in serum supplemented media normally require adjustment period in order to successfully propagate in Serum-Free Hybridoma Medium. Adjustment periods can be shortened when adjustment methods are properly administered.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSF 401 \u003c\/strong\u003econtains D-glucose, HEPES, sodium bicarbonate, L-glutamine, Pluronic® F-68, and thus does not require further supplements. \u003cstrong\u003eSF 401\u003c\/strong\u003e vary in their compositions and consequently yield different effects on different cell lines. The optimal product for a certain cell type can only be confirmed through direct application. \u003cstrong\u003eSF 401 \u003c\/strong\u003econtains the minimal amount of ingredients of animal origin, and is therefore appropriate for the study and production of medical quality recombinant proteins, which is the final product of cell cultivation. In selecting the appropriate culture environment of Serum-Free Medium, (1) the origin of the cell host (the kind of cell), (2) the type of culture container to be used (tissue flask, multiplayer flasks, roller bottle, spinner flasks, or bioreactors), and (3) the characteristics of the cell host, are important factors to be considered.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eStorage\/Stability\u003cbr\u003e\u003c\/strong\u003eThe Serum-Free medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and\/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.\u003c\/p\u003e\n\u003cp\u003e \u003cstrong\u003eInstructions for Use\u003cbr\u003e\u003c\/strong\u003e(1) Nutritional requirements can change in accordance with each gene expression system, (2) recombinant protein expression can be increased by supplementing polyamine supplement (\u003cstrong\u003eLS 018-01\u003c\/strong\u003e), fatty acid supplement (\u003cstrong\u003eLS 017-01\u003c\/strong\u003e), sodium butyrate (\u003cstrong\u003eLS 033-01\u003c\/strong\u003e), (3) when using a CO\u003csub\u003e2\u003c\/sub\u003e incubator, the pH of the cell culture should be adjusted by controlling the NaHCO\u003csub\u003e3 \u003c\/sub\u003econcentration, (4) when using a spinner flask, leave the cover 1\/4 open to allow aeration.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eBiological Performance Characteristics\u003cbr\u003e\u003c\/strong\u003eThe growth-promoting capacities of the Serum-Free Hybridoma media are tested in a liquid medium using Hybridoma cell. Growth rates are examined through three subculture. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePrecautions\u003cbr\u003e\u003c\/strong\u003eSerum-Free Hybridoma media are only used \u003cem\u003eIn Vitro, \u003c\/em\u003eand are sensitive to light and heat. Adhering to the following suggestions can increase the product shelf life. (1) Limit light exposure, (2) minimize its exposure to room temperature (3) store in a darkroom at 2~8°C, (4) use in small amounts at a time.\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e","brand":"Welgene","offers":[{"title":"1L","offer_id":43540255310041,"sku":null,"price":108000.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/products\/SF401.jpg?v=1671495424"},{"product_id":"serum-free-cho-medium-sf486","title":"Serum-Free CHO Medium  (SF486)","description":"\u003ctable width=\"100%\" style=\"border-collapse: collapse;\" data-mce-style=\"border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid black; width: 46.6089%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 46.6089%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/SF_CHO_Medium_SF486.pdf?v=1681950360\" target=\"_blank\"\u003e\u003cstrong\u003e제품 설명서 (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd colspan=\"2\" style=\"border: 1px solid black; width: 53.3911%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 53.3911%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/Serum-Free_CHO_Medium_SF486_GHS_MSDS.pdf?v=1682337641\" rel=\"noopener noreferrer\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSerum-Free CHO Medium, Liquid\u003cbr\u003e\u003c\/strong\u003eWith 4500 mg\/L D-glucose\u003cbr\u003eWith 7.5 mM HEPES\u003cbr\u003eWith sodium bicarbonate\u003cbr\u003eWith L-glutamine\u003cbr\u003eWith Pluronic® F68\u003cbr\u003eFor the suspension culture of CHO cell\u003c\/p\u003e\n\u003cp\u003eCatalog Number \u003cstrong\u003eSF 486\u003cbr\u003e\u003c\/strong\u003eStorage Temperature 2~8°C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003cbr\u003e\u003c\/strong\u003eSerum-Free CHO Media were developed to support the cell growth of Chinese Hamster Ovary cells in suspension cultures. Serum-Free CHO Medium differs from normal (serum supplemented) media in its amino acid, vitamin, and mineral composition, and includes peptides, fatty acids, and growth factors as substitutes to serum. When transplanting CHO cells that had been formerly cultured in a serum supplemented medium, direct adaptation or sequential adaptation should be administered for the appropriate amount of time. When trypsin is used in sub-cultivation of cells in an adherent culture, trypsin inhibitor is supplemented in place for serum for neutralizing effects. A centrifuge process followed by cell cleansing eliminates trypsin from the formulation. The absence of trypsin allows culture media to be transferred into a new container and continue to sustain cell growth with added Serum-Free Medium. If needed, the medium is to be centrifuged at 800 rpm for 5 minutes prior to transfer.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSF 486 \u003c\/strong\u003econtains 4500 mg\/L D-glucose, 7.5 mM HEPES, L-glutamine, and sodium bicarbonate. Pluronic® F-68 is supplemented to prevent damage to CHO cells in a suspension culture, along with hypoxanthine and thymidine. \u003cstrong\u003eSF 486\u003c\/strong\u003e vary in their compositions and consequently yield different effects on different cell lines. The optimal product for a certain cell type can only be confirmed through direct application. \u003cstrong\u003eSF 486 \u003c\/strong\u003econtains the minimal amount of reagents of animal origin, thus making them appropriate for the research and production of medical quality recombinant proteins. In selecting the appropriate culture environment of Serum-Free Medium, (1) the origin of the cell host (the kind of cell), (2) the type of culture container to be used (tissue flask, multiplayer flasks, roller bottle, spinner flasks, or bioreactors), and (3) the characteristics of the CHO cell line is important factors to be considered.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eStorage\/Stability\u003cbr\u003e\u003c\/strong\u003eThe Serum-Free medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and\/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eInstructions for Use\u003cbr\u003e\u003c\/strong\u003e(1) \u003cstrong\u003eSF 486\u003c\/strong\u003e is generally supplemented with 2~8 mM of L-glutamine (\u003cstrong\u003eLS 002-01\u003c\/strong\u003e), (2) nutritional requirements can be altered according to the gene expression system, (3) the concentration of D-glucose (\u003cstrong\u003eLS 001-01\u003c\/strong\u003e or \u003cstrong\u003eLS 001-02\u003c\/strong\u003e) can be adjusted up to 6.8 g\/L, (4) polyamine supplement (\u003cstrong\u003eLS 018-01\u003c\/strong\u003e), fatty acid supplement (\u003cstrong\u003eLS 017-01\u003c\/strong\u003e), sodium butyrate (\u003cstrong\u003eLS 033-01\u003c\/strong\u003e) is recommended for encouraging the expression of recombinant proteins, (5) when cultivating in a CO\u003csub\u003e2\u003c\/sub\u003e incubator, adjustment of pH should be made by altering NaHCO\u003csub\u003e3 \u003c\/sub\u003econcentration, (6) when preparing a suspension culture in a spinner flask, allow 1\/4 of the tray to be open to ensure aeration.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBiological Performance Characteristics\u003cbr\u003e\u003c\/strong\u003eThe growth-promoting capacities of the Serum-Free CHO media are tested in a liquid medium using CHO cell. Growth rates are examined through three subculture. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePrecautions\u003cbr\u003e\u003c\/strong\u003eSerum-Free CHO media are only used \u003cem\u003eIn Vitro, \u003c\/em\u003eand are sensitive to light and heat. Adhering to the following suggestions can increase the product shelf life. (1) Limit light exposure, (2) avoid keeping at room temperature (around 37°C), (3) store in a darkroom at 2~8°C, (4) use in small amounts at a time.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAdaptation of CHO cells to Serum-Free media\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA sequential adaptation protocol may be necessary if direct adaptation does not work.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e       Direct Adaptation (1)\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eIf CHO cell confluence reaches more than 80% in serum supplemented medium, remove culture medium.\u003c\/li\u003e\n\u003cli\u003eCell, remove using Trypsin or Trypsin-EDTA for 2~3 minutes at 37°C and cell neutralizes using serum supplemented medium. And then remove supernatant by centrifugation cells at 800 rpm for 5 minutes.\u003c\/li\u003e\n\u003cli\u003eCell suspended using Serum-Free medium. And remove supernatant by centrifugation cells at 800 rpm for 5 minutes.\u003c\/li\u003e\n\u003cli\u003eResuspend cells to a viable cell density of 2~5X10\u003csup\u003e5\u003c\/sup\u003e cells\/mL in Serum-Free medium. Incubate the cells in spinner flask at 40 rpm.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cstrong\u003e      \u003c\/strong\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003eDirect Adaptation (2)\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eIf CHO cell confluence reaches more than 90% serum supplemented medium, convert culture medium into Serum-Free medium.\u003c\/li\u003e\n\u003cli\u003eGather and culture suspension cell in T-flask of one while subculture the cells using Serum-Free medium.\u003c\/li\u003e\n\u003cli\u003eConfirmed growth cell is subculture. Resuspend cells to a viable cell density of 2~5X10\u003csup\u003e5\u003c\/sup\u003e cells\/mL in Serum-Free medium. Incubate the cells in spinner flask at 40 rpm.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cstrong\u003e       \u003c\/strong\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003eSequential Adaptation\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eIf CHO cell confluence reaches more than 80% serum supplemented medium, remove cell using Trypsin or Trypsin-EDTA. Incubate cells at viable cell density of 3~4X10\u003csup\u003e5\u003c\/sup\u003e cells\/mL in a 75:25 (v\/v) mixture of serum supplemented:Serum-free medium.\u003c\/li\u003e\n\u003cli\u003eRepeat subculture 2~4 times in same conditions and monitor the culture until the density reaches 90 % viable cells\/mL compare with existing culture. Then subculture at viable cell density of 3~4X10\u003csup\u003e5\u003c\/sup\u003e cells\/mL into a 50:50 (v\/v) mixture of serum supplemented: Serum-Free medium.\u003c\/li\u003e\n\u003cli\u003eRepeat process 2, do subculture into a 25:75 (v\/v) mixture of serum supplemented:Serum-Free medium.\u003c\/li\u003e\n\u003cli\u003eRepeat process 2, do subculture into a Serum-Free medium only.\u003c\/li\u003e\n\u003cli\u003eIf cell growth is confirmed, cell freeze using freezing medium (contained Serum-Free medium) and examines cell activity after thawing.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e","brand":"Welgene","offers":[{"title":"1L","offer_id":43540260258009,"sku":null,"price":108000.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/products\/SF486.jpg?v=1671495450"}],"url":"https:\/\/en.welgene.com\/ko\/collections\/serum-free-and-protein-free-media.oembed","provider":"Welgene","version":"1.0","type":"link"}