{"title":"Fetal Bovine Sera (FBS)","description":"","products":[{"product_id":"s001-04-stem-cell-qualifiedmsc-tested","title":"FBS, Stem Cell Qualified (S001-04)","description":"\u003ctable width=\"100%\" style=\"border-collapse: collapse;\" data-mce-style=\"border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid black; width: 46.6089%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 46.6089%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS.pdf?v=1682397400\" rel=\"noopener noreferrer\" target=\"_blank\"\u003e\u003cstrong\u003e제품 설명서 (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd colspan=\"2\" style=\"border: 1px solid black; width: 53.3911%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 53.3911%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS_S001-04_GHS_MSDS.pdf?v=1682391595\" rel=\"noopener noreferrer\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/STEM1_2048x2048.jpg?v=1667465943\" alt=\"\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/STEM1_2048x2048.jpg?v=1667465943\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/STEM2_2048x2048.jpg?v=1667465949\" alt=\"\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/STEM2_2048x2048.jpg?v=1667465949\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProper storage and thawing method of FBS\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003col data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAfter FBS thawing, why does clots of fleece shape appear? And how affect it to cell culture?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDuring FBS thawing, the most important thing is to shake slowly every 5~10 minutes until thawed all, minimizing bubbles. If the process does not work correctly, concentration gradient with insoluble crystals of fleece shape(cryoprecipitates) can be generated result from imbalance of salt and protein.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe cryoprecipitates is little effect on cell culture. In particular, thawed FBS should not be refrigerated and if you don’t follow the precautions, the clots can be caused a lot of further. Because of this, aliquots of thawing must be frozen keep. The cryoprecipitates is not a defect or fault of product.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003col start=\"2\" data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eWhat is proper storage and use method of FBS?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e1) Proper FBS thawing process(for minimizing deposits)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eWhile FBS thawing, shake it slowly every 5 ~ 10  minutes minimizing bubbles for breaking concentration gradient.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFill water in water bath almost to top of FBS(except cap) and raise temperature of water both by 37\u003c\/span\u003e℃\u003cspan data-mce-fragment=\"1\"\u003e..\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003ePut frozen FBS in water bath and thaw it by shaking slowly ever 5~10 minutes until breaking concentration gradient.  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eAfter thawing all, take out and spray 70% alcohol on the surface of bottle.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eDivide FBS into 20~100ml in clean bench.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFreeze divided FBS as soon as possible. And keep FBS in refrigerator if it is used in a day or two. But keep in mind FBS kept in refrigerator can generate more clots than one in freezer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e If Heat-Inactivation process is needed, proceed as follows (in case of buying products that Heat-Inactivation completed, this process is unnecessary)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e   - Put thawed FBS in 56℃\u003cspan data-mce-fragment=\"1\"\u003e water bath and proceed Heat-Inactivation for 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e  - Shake FBS slowly every 5~10 minutes minimizing bubbles and not making concentration gradient like thawing process. If this Heat-inactivation proceeded irregularly, lots of clots can be generated than thawing process.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e2) Must storage FBS in a freezer without automatic frost-free function. (Avoid storage in a home refrigerator\/freezer, which has frost-free function.)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cu data-mce-fragment=\"1\"\u003eMust Storage in a freezer without automatic frost-free function for preventing deterioration of FBS from temperature change.\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003c\/u\u003e A freezer with automatic frost-free function change the temperature automatically for preventing frost, so it can cause reduce the quality of FBS by freezing and thawing the surface of product repeatedly. And give attention that product bottle can be broken in temperature.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e3) Minimize freezing-thawing process\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTemperature change from freezing-thawing process destroys protein, growth factor, hormone and other nutrient. Thus,\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cu data-mce-fragment=\"1\"\u003eit is necessary to minimize freezing-thawing process.\u003c\/u\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eAfter first thawing, divide FBS into amount used to once or twice(20~100ml) and frozen. This can reduce iterative freezing-thawing process and thawing hours. At this time attention not to exceed maximum volume 40ml in 50ml conical tube and 90ml in 100ml glass bottle considering of FBS expands as it freezes.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cstyle\u003e\n        .product-table {\n            width: 100%;\n            border-collapse: collapse;\n            background-color: #f9f9f9;\n        }\n        .product-table th, .product-table td {\n            padding: 10px;\n            text-align: center;\n            border: none;\n            white-space: nowrap;\n        }\n        .product-table th {\n            background-color: #f2f2f2;\n            white-space: nowrap;\n        }\n        .product-table .product-row {\n            background-color: #ffffff;\n        }\n        .product-table .product-name, .product-table .description {\n            text-align: left;\n            white-space: normal;\n        }\n    \u003c\/style\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003ctable class=\"product-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align: left;\" class=\"product-name\"\u003eProduct Name\u003c\/th\u003e\n\u003cth style=\"text-align: left;\" class=\"description\"\u003eDescription\u003c\/th\u003e\n\u003cth style=\"text-align: center;\" class=\"catno-cell\"\u003eCat. No.\u003c\/th\u003e\n\u003cth style=\"text-align: center;\" class=\"storage-cell\"\u003eRecommended Storage Temp.\u003c\/th\u003e\n\u003cth style=\"text-align: center;\" class=\"size-cell\"\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"product-row\"\u003e\n\u003ctd style=\"text-align: left;\" class=\"product-name\"\u003eFetal Bovine Serum (FBS) Premium\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\" class=\"description\"\u003ePremium\u003cbr\u003e0.1 μm sterile-filtered\u003cbr\u003eLow endotoxin\u003cbr\u003eLow hemoglobin\u003cbr\u003ePerformance, mycoplasma, virus, bacteriophage, and endotoxin tested.\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"catno-cell\"\u003eS 001-01\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"storage-cell\"\u003eStore at less than -20°C\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"size-cell\"\u003e100ML, 500ML\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"product-row\"\u003e\n\u003ctd style=\"text-align: left;\" class=\"product-name\"\u003eFetal Bovine Serum (FBS) Certified\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\" class=\"description\"\u003eCertified\u003cbr\u003e0.1 μm sterile-filtered\u003cbr\u003eLow endotoxin\u003cbr\u003eLow hemoglobin\u003cbr\u003ePerformance, mycoplasma, virus, bacteriophage, and endotoxin tested.\u003cbr\u003ePerformance, mycoplasma, virus, bacteriophage, and endotoxin tested.\u003cbr\u003eTested for the ability to sustain undifferentiated cellular morphology of embryonic stem cells.\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"catno-cell\"\u003eS 001-07\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"storage-cell\"\u003eStore at less than -20°C\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"size-cell\"\u003e100ML, 500ML\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"product-row\"\u003e\n\u003ctd style=\"text-align: left;\" class=\"product-name\"\u003eFetal Bovine Serum (FBS), Stem Cell Qualified (MSC tested)\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\" class=\"description\"\u003ePerformance, mycoplasma, virus, bacteriophage, and endotoxin tested.\u003cbr\u003eTested for the ability to sustain undifferentiated cellular morphology of embryonic stem cells.\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"catno-cell\"\u003eS 001-04\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"storage-cell\"\u003eStore at less than -20°C\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"size-cell\"\u003e100ML, 500ML\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"product-row\"\u003e\n\u003ctd style=\"text-align: left;\" class=\"product-name\"\u003eBovine Calf Serum (BCS)\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\" class=\"description\"\u003eUSA origin\u003cbr\u003eCell culture, mycoplasma, virus, and endotoxin tested.\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"catno-cell\"\u003eS 003-01\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"storage-cell\"\u003eStore at less than -20°C\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"size-cell\"\u003e100ML, 500ML\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComparison of FBS\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cstyle\u003e\n        .comparison-table {\n            width: 100%;\n            border-collapse: collapse;\n            background-color: #ffffff;\n        }\n        .comparison-table th, .comparison-table td {\n            padding: 10px;\n            text-align: center;\n            border: none;\n            white-space: nowrap;\n        }\n        .comparison-table th {\n            background-color: #f2f2f2;\n            white-space: nowrap;\n        }\n    \u003c\/style\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003ctable style=\"border: none; width: 112%;\" class=\"comparison-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 30.8277%;\"\u003eCat. No.\u003c\/th\u003e\n\u003cth style=\"width: 15.5761%;\"\u003eS 001-01\u003c\/th\u003e\n\u003cth style=\"width: 15.5761%;\"\u003eS001-07\u003c\/th\u003e\n\u003cth style=\"width: 21.5794%;\"\u003eS 001-04\u003c\/th\u003e\n\u003cth style=\"width: 16.5496%;\"\u003eS 003-01\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eSource\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003eBovine fetus\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003eBovine fetus\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003eBovine fetus\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003eBovine calves\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eCountry\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003eUSA\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003eUSA\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003eUSA\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003eUSA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eSterility test\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eMycoplasma test\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eBacteriophage test\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eEndotoxin (EU\/ml)\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e≤ 10\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e≤ 10\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e≤ 10\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e≤ 100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003ePerformance\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eHeat-inactivation\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eHemoglobin (mg%)\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e≤ 20\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e≤ 20\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e≤ 20\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e≤ 20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eOsmolality (mOsm\/kg H\u003csub\u003e2\u003c\/sub\u003eO)\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e300 ± 10%\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e300 ± 10%\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e300 ± 10%\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e290 ± 20%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003epH at RT\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e7.3 ± 0.7\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e7.3 ± 0.7\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e7.3 ± 0.7\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e7.5 ± 0.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eCharacteristics\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003eStem cell qualified\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Welgene","offers":[{"title":"100ML","offer_id":43537023238361,"sku":"","price":200000.0,"currency_code":"KRW","in_stock":true},{"title":"500ML","offer_id":43096781357273,"sku":null,"price":800000.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/products\/S001-04.jpg?v=1672118816"},{"product_id":"fetal-bovine-serum-fbs-premium","title":"FBS, Premium (S001-01)","description":"\u003ctable style=\"border-collapse: collapse;\" width=\"100%\" data-mce-style=\"border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid black; width: 46.6089%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 46.6089%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS.pdf?v=1682397400\" rel=\"noopener noreferrer\" target=\"_blank\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS.pdf?v=1682397400\"\u003e\u003cstrong\u003e제품 설명서 (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid black; width: 53.3911%; text-align: center;\" colspan=\"2\" data-mce-style=\"border: 1px solid black; width: 53.3911%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS_S001-01_GHS_MSDS.pdf?v=1682391595\" rel=\"noopener noreferrer\" target=\"_blank\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS_S001-01_GHS_MSDS.pdf?v=1682391595\"\u003e\u003cstrong\u003eMSDS (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProper storage and thawing method of FBS\u003c\/strong\u003e\u003c\/p\u003e\n\u003col data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAfter FBS thawing, why does clots of fleece shape appear? And how affect it to cell culture?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDuring FBS thawing, the most important thing is to shake slowly every 5~10 minutes until thawed all, minimizing bubbles. If the process does not work correctly, concentration gradient with insoluble crystals of fleece shape(cryoprecipitates) can be generated result from imbalance of salt and protein.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe cryoprecipitates is little effect on cell culture. In particular, thawed FBS should not be refrigerated and if you don’t follow the precautions, the clots can be caused a lot of further. Because of this, aliquots of thawing must be frozen keep. The cryoprecipitates is not a defect or fault of product.\u003c\/p\u003e\n\u003col data-mce-fragment=\"1\" start=\"2\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e What is proper storage and use method of FBS?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e1) Proper FBS thawing process(for minimizing deposits)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eWhile FBS thawing, shake it slowly every 5 ~ 10  minutes minimizing bubbles for breaking concentration gradient.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★ \u003cspan data-mce-fragment=\"1\"\u003eFill water in water bath almost to top of FBS(except cap) and raise temperature of water both by 37\u003c\/span\u003e℃\u003cspan data-mce-fragment=\"1\"\u003e..\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★ \u003cspan data-mce-fragment=\"1\"\u003ePut frozen FBS in water bath and thaw it by shaking slowly ever 5~10 minutes until breaking concentration gradient.  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★ \u003cspan data-mce-fragment=\"1\"\u003eAfter thawing all, take out and spray 70% alcohol on the surface of bottle.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★ \u003cspan data-mce-fragment=\"1\"\u003eDivide FBS into 20~100ml in clean bench.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★ \u003cspan data-mce-fragment=\"1\"\u003eFreeze divided FBS as soon as possible. And keep FBS in refrigerator if it is used in a day or two. But keep in mind FBS kept in refrigerator can generate more clots than one in freezer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e If Heat-Inactivation process is needed, proceed as follows (in case of buying products that Heat-Inactivation completed, this process is unnecessary)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e   - Put thawed FBS in 56℃\u003cspan data-mce-fragment=\"1\"\u003e water bath and proceed Heat-Inactivation for 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e  - Shake FBS slowly every 5~10 minutes minimizing bubbles and not making concentration gradient like thawing process. If this Heat-inactivation proceeded irregularly, lots of clots can be generated than thawing process.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003cstrong data-mce-fragment=\"1\"\u003e2) Must storage FBS in a freezer without automatic frost-free function. (Avoid storage in a home refrigerator\/freezer, which has frost-free function.)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eMust Storage in a freezer without automatic frost-free function for preventing deterioration of FBS from temperature change.  A freezer with automatic frost-free function change the temperature automatically for preventing frost, so it can cause reduce the quality of FBS by freezing and thawing the surface of product repeatedly. And give attention that product bottle can be broken in temperature.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003cstrong data-mce-fragment=\"1\"\u003e3) Minimize freezing-thawing process\u003cbr\u003e\u003c\/strong\u003eTemperature change from freezing-thawing process destroys protein, growth factor, hormone and other nutrient. Thus, \u003cu data-mce-fragment=\"1\"\u003eit is necessary to minimize freezing-thawing process.\u003c\/u\u003e After first thawing, divide FBS into amount used to once or twice(20~100ml) and frozen. This can reduce iterative freezing-thawing process and thawing hours. At this time attention not to exceed maximum volume 40ml in 50ml conical tube and 90ml in 100ml glass bottle considering of FBS expands as it freezes.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cstyle\u003e\n        .product-table {\n            width: 100%;\n            border-collapse: collapse;\n            background-color: #f9f9f9;\n        }\n        .product-table th, .product-table td {\n            padding: 10px;\n            text-align: center;\n            border: none;\n            white-space: nowrap;\n        }\n        .product-table th {\n            background-color: #f2f2f2;\n            white-space: nowrap;\n        }\n        .product-table .product-row {\n            background-color: #ffffff;\n        }\n        .product-table .product-name, .product-table .description {\n            text-align: left;\n            white-space: normal;\n        }\n    \u003c\/style\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003ctable class=\"product-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align: left;\" class=\"product-name\"\u003eProduct Name\u003c\/th\u003e\n\u003cth style=\"text-align: left;\" class=\"description\"\u003eDescription\u003c\/th\u003e\n\u003cth style=\"text-align: center;\" class=\"catno-cell\"\u003eCat. No.\u003c\/th\u003e\n\u003cth style=\"text-align: center;\" class=\"storage-cell\"\u003eRecommended Storage Temp.\u003c\/th\u003e\n\u003cth style=\"text-align: center;\" class=\"size-cell\"\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"product-row\"\u003e\n\u003ctd style=\"text-align: left;\" class=\"product-name\"\u003eFetal Bovine Serum (FBS) Premium\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\" class=\"description\"\u003ePremium\u003cbr\u003e0.1 μm sterile-filtered\u003cbr\u003eLow endotoxin\u003cbr\u003eLow hemoglobin\u003cbr\u003ePerformance, mycoplasma, virus, bacteriophage, and endotoxin tested.\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"catno-cell\"\u003eS 001-01\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"storage-cell\"\u003eStore at less than -20°C\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"size-cell\"\u003e100ML, 500ML\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"product-row\"\u003e\n\u003ctd style=\"text-align: left;\" class=\"product-name\"\u003eFetal Bovine Serum (FBS) Certified\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\" class=\"description\"\u003eCertified\u003cbr\u003e0.1 μm sterile-filtered\u003cbr\u003eLow endotoxin\u003cbr\u003eLow hemoglobin\u003cbr\u003ePerformance, mycoplasma, virus, bacteriophage, and endotoxin tested.\u003cbr\u003ePerformance, mycoplasma, virus, bacteriophage, and endotoxin tested.\u003cbr\u003eTested for the ability to sustain undifferentiated cellular morphology of embryonic stem cells.\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"catno-cell\"\u003eS 001-07\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"storage-cell\"\u003eStore at less than -20°C\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"size-cell\"\u003e100ML, 500ML\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"product-row\"\u003e\n\u003ctd style=\"text-align: left;\" class=\"product-name\"\u003eFetal Bovine Serum (FBS), Stem Cell Qualified (MSC tested)\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\" class=\"description\"\u003ePerformance, mycoplasma, virus, bacteriophage, and endotoxin tested.\u003cbr\u003eTested for the ability to sustain undifferentiated cellular morphology of embryonic stem cells.\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"catno-cell\"\u003eS 001-04\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"storage-cell\"\u003eStore at less than -20°C\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"size-cell\"\u003e100ML, 500ML\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"product-row\"\u003e\n\u003ctd style=\"text-align: left;\" class=\"product-name\"\u003eBovine Calf Serum (BCS)\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\" class=\"description\"\u003eUSA origin\u003cbr\u003eCell culture, mycoplasma, virus, and endotoxin tested.\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"catno-cell\"\u003eS 003-01\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"storage-cell\"\u003eStore at less than -20°C\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"size-cell\"\u003e100ML, 500ML\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComparison of FBS\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cstyle\u003e\n        .comparison-table {\n            width: 100%;\n            border-collapse: collapse;\n            background-color: #ffffff;\n        }\n        .comparison-table th, .comparison-table td {\n            padding: 10px;\n            text-align: center;\n            border: none;\n            white-space: nowrap;\n        }\n        .comparison-table th {\n            background-color: #f2f2f2;\n            white-space: nowrap;\n        }\n    \u003c\/style\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003ctable style=\"border: none; width: 112%;\" class=\"comparison-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 30.8277%;\"\u003eCat. No.\u003c\/th\u003e\n\u003cth style=\"width: 15.5761%;\"\u003eS 001-01\u003c\/th\u003e\n\u003cth style=\"width: 15.5761%;\"\u003eS001-07\u003c\/th\u003e\n\u003cth style=\"width: 21.5794%;\"\u003eS 001-04\u003c\/th\u003e\n\u003cth style=\"width: 16.5496%;\"\u003eS 003-01\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eSource\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003eBovine fetus\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003eBovine fetus\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003eBovine fetus\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003eBovine calves\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eCountry\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003eUSA\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003eUSA\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003eUSA\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003eUSA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eSterility test\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eMycoplasma test\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eBacteriophage test\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eEndotoxin (EU\/ml)\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e≤ 10\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e≤ 10\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e≤ 10\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e≤ 100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003ePerformance\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eHeat-inactivation\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eHemoglobin (mg%)\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e≤ 20\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e≤ 20\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e≤ 20\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e≤ 20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eOsmolality (mOsm\/kg H\u003csub\u003e2\u003c\/sub\u003eO)\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e300 ± 10%\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e300 ± 10%\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e300 ± 10%\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e290 ± 20%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003epH at RT\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e7.3 ± 0.7\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e7.3 ± 0.7\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e7.3 ± 0.7\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e7.5 ± 0.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eCharacteristics\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003eStem cell qualified\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Welgene","offers":[{"title":"100ML","offer_id":43117836337369,"sku":null,"price":164000.0,"currency_code":"KRW","in_stock":true},{"title":"500ML","offer_id":43117836370137,"sku":null,"price":655000.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/S001-01_best.jpg?v=1710815104"},{"product_id":"fetal-bovine-serum-fbs-certified","title":"FBS,  Certified (S001-07)","description":"\u003ctable style=\"border-collapse: collapse;\" width=\"100%\" data-mce-style=\"border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid black; width: 46.6089%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 46.6089%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS.pdf?v=1682397400\" target=\"_blank\"\u003e\u003cstrong\u003e제품 설명서 (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid black; width: 53.3911%; text-align: center;\" colspan=\"2\" data-mce-style=\"border: 1px solid black; width: 53.3911%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS_S001-07_GHS_MSDS.pdf?v=1682391595\" rel=\"noopener noreferrer\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProper storage and thawing method of FBS\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003col data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAfter FBS thawing, why does clots of fleece shape appear? And how affect it to cell culture?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDuring FBS thawing, the most important thing is to shake slowly every 5~10 minutes until thawed all, minimizing bubbles. If the process does not work correctly, concentration gradient with insoluble crystals of fleece shape(cryoprecipitates) can be generated result from imbalance of salt and protein.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe cryoprecipitates is little effect on cell culture. In particular, thawed FBS should not be refrigerated and if you don’t follow the precautions, the clots can be caused a lot of further. Because of this, aliquots of thawing must be frozen keep. The cryoprecipitates is not a defect or fault of product.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003col start=\"2\" data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eWhat is proper storage and use method of FBS?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e1) Proper FBS thawing process(for minimizing deposits)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eWhile FBS thawing, shake it slowly every 5 ~ 10  minutes minimizing bubbles for breaking concentration gradient.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFill water in water bath almost to top of FBS(except cap) and raise temperature of water both by 37\u003c\/span\u003e℃\u003cspan data-mce-fragment=\"1\"\u003e..\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003ePut frozen FBS in water bath and thaw it by shaking slowly ever 5~10 minutes until breaking concentration gradient.  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eAfter thawing all, take out and spray 70% alcohol on the surface of bottle.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eDivide FBS into 20~100ml in clean bench.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFreeze divided FBS as soon as possible. And keep FBS in refrigerator if it is used in a day or two. But keep in mind FBS kept in refrigerator can generate more clots than one in freezer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e If Heat-Inactivation process is needed, proceed as follows (in case of buying products that Heat-Inactivation completed, this process is unnecessary)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e   - Put thawed FBS in 56℃\u003cspan data-mce-fragment=\"1\"\u003e water bath and proceed Heat-Inactivation for 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e  - Shake FBS slowly every 5~10 minutes minimizing bubbles and not making concentration gradient like thawing process. If this Heat-inactivation proceeded irregularly, lots of clots can be generated than thawing process.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e2) Must storage FBS in a freezer without automatic frost-free function. (Avoid storage in a home refrigerator\/freezer, which has frost-free function.)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cu data-mce-fragment=\"1\"\u003eMust Storage in a freezer without automatic frost-free function for preventing deterioration of FBS from temperature change.\u003cspan\u003e \u003c\/span\u003e\u003c\/u\u003e A freezer with automatic frost-free function change the temperature automatically for preventing frost, so it can cause reduce the quality of FBS by freezing and thawing the surface of product repeatedly. And give attention that product bottle can be broken in temperature.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e3) Minimize freezing-thawing process\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTemperature change from freezing-thawing process destroys protein, growth factor, hormone and other nutrient. Thus,\u003cspan\u003e \u003c\/span\u003e\u003cu data-mce-fragment=\"1\"\u003eit is necessary to minimize freezing-thawing process.\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003eAfter first thawing, divide FBS into amount used to once or twice(20~100ml) and frozen. This can reduce iterative freezing-thawing process and thawing hours. At this time attention not to exceed maximum volume 40ml in 50ml conical tube and 90ml in 100ml glass bottle considering of FBS expands as it freezes.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cstyle\u003e\n        .product-table {\n            width: 100%;\n            border-collapse: collapse;\n            background-color: #f9f9f9;\n        }\n        .product-table th, .product-table td {\n            padding: 10px;\n            text-align: center;\n            border: none;\n            white-space: nowrap;\n        }\n        .product-table th {\n            background-color: #f2f2f2;\n            white-space: nowrap;\n        }\n        .product-table .product-row {\n            background-color: #ffffff;\n        }\n        .product-table .product-name, .product-table .description {\n            text-align: left;\n            white-space: normal;\n        }\n    \u003c\/style\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003ctable class=\"product-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align: left;\" class=\"product-name\"\u003eProduct Name\u003c\/th\u003e\n\u003cth style=\"text-align: left;\" class=\"description\"\u003eDescription\u003c\/th\u003e\n\u003cth style=\"text-align: center;\" class=\"catno-cell\"\u003eCat. No.\u003c\/th\u003e\n\u003cth style=\"text-align: center;\" class=\"storage-cell\"\u003eRecommended Storage Temp.\u003c\/th\u003e\n\u003cth style=\"text-align: center;\" class=\"size-cell\"\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"product-row\"\u003e\n\u003ctd style=\"text-align: left;\" class=\"product-name\"\u003eFetal Bovine Serum (FBS) Premium\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\" class=\"description\"\u003ePremium\u003cbr\u003e0.1 μm sterile-filtered\u003cbr\u003eLow endotoxin\u003cbr\u003eLow hemoglobin\u003cbr\u003ePerformance, mycoplasma, virus, bacteriophage, and endotoxin tested.\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"catno-cell\"\u003eS 001-01\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"storage-cell\"\u003eStore at less than -20°C\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"size-cell\"\u003e100ML, 500ML\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"product-row\"\u003e\n\u003ctd style=\"text-align: left;\" class=\"product-name\"\u003eFetal Bovine Serum (FBS) Certified\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\" class=\"description\"\u003eCertified\u003cbr\u003e0.1 μm sterile-filtered\u003cbr\u003eLow endotoxin\u003cbr\u003eLow hemoglobin\u003cbr\u003ePerformance, mycoplasma, virus, bacteriophage, and endotoxin tested.\u003cbr\u003ePerformance, mycoplasma, virus, bacteriophage, and endotoxin tested.\u003cbr\u003eTested for the ability to sustain undifferentiated cellular morphology of embryonic stem cells.\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"catno-cell\"\u003eS 001-07\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"storage-cell\"\u003eStore at less than -20°C\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"size-cell\"\u003e100ML, 500ML\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"product-row\"\u003e\n\u003ctd style=\"text-align: left;\" class=\"product-name\"\u003eFetal Bovine Serum (FBS), Stem Cell Qualified (MSC tested)\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\" class=\"description\"\u003ePerformance, mycoplasma, virus, bacteriophage, and endotoxin tested.\u003cbr\u003eTested for the ability to sustain undifferentiated cellular morphology of embryonic stem cells.\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"catno-cell\"\u003eS 001-04\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"storage-cell\"\u003eStore at less than -20°C\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"size-cell\"\u003e100ML, 500ML\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"product-row\"\u003e\n\u003ctd style=\"text-align: left;\" class=\"product-name\"\u003eBovine Calf Serum (BCS)\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\" class=\"description\"\u003eUSA origin\u003cbr\u003eCell culture, mycoplasma, virus, and endotoxin tested.\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"catno-cell\"\u003eS 003-01\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"storage-cell\"\u003eStore at less than -20°C\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" class=\"size-cell\"\u003e100ML, 500ML\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComparison of FBS\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cstyle\u003e\n        .comparison-table {\n            width: 100%;\n            border-collapse: collapse;\n            background-color: #ffffff;\n        }\n        .comparison-table th, .comparison-table td {\n            padding: 10px;\n            text-align: center;\n            border: none;\n            white-space: nowrap;\n        }\n        .comparison-table th {\n            background-color: #f2f2f2;\n            white-space: nowrap;\n        }\n    \u003c\/style\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003ctable style=\"border: none; width: 112%;\" class=\"comparison-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 30.8277%;\"\u003eCat. No.\u003c\/th\u003e\n\u003cth style=\"width: 15.5761%;\"\u003eS 001-01\u003c\/th\u003e\n\u003cth style=\"width: 15.5761%;\"\u003eS001-07\u003c\/th\u003e\n\u003cth style=\"width: 21.5794%;\"\u003eS 001-04\u003c\/th\u003e\n\u003cth style=\"width: 16.5496%;\"\u003eS 003-01\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eSource\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003eBovine fetus\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003eBovine fetus\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003eBovine fetus\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003eBovine calves\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eCountry\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003eUSA\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003eUSA\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003eUSA\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003eUSA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eSterility test\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eMycoplasma test\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eBacteriophage test\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eEndotoxin (EU\/ml)\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e≤ 10\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e≤ 10\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e≤ 10\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e≤ 100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003ePerformance\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003ePass\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eHeat-inactivation\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eHemoglobin (mg%)\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e≤ 20\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e≤ 20\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e≤ 20\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e≤ 20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eOsmolality (mOsm\/kg H\u003csub\u003e2\u003c\/sub\u003eO)\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e300 ± 10%\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e300 ± 10%\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e300 ± 10%\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e290 ± 20%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003epH at RT\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e7.3 ± 0.7\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e7.3 ± 0.7\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003e7.3 ± 0.7\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e7.5 ± 0.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.8277%;\"\u003eCharacteristics\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 15.5761%;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 21.5794%;\"\u003eStem cell qualified\u003c\/td\u003e\n\u003ctd style=\"width: 16.5496%;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Welgene","offers":[{"title":"100ML","offer_id":43117942669529,"sku":null,"price":155000.0,"currency_code":"KRW","in_stock":true},{"title":"500ML","offer_id":43117942636761,"sku":null,"price":625000.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/S001-07_best.jpg?v=1710815138"},{"product_id":"fetal-bovine-serum-fbs-heat-inactivated-1","title":"FBS Premium Heat-Inactivated (S101-01)","description":"\u003ctable style=\"border-collapse: collapse;\" width=\"100%\" data-mce-style=\"border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid black; width: 46.6089%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 46.6089%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS.pdf?v=1682397400\" target=\"_blank\"\u003e\u003cstrong\u003e제품 설명서 (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid black; width: 53.3911%; text-align: center;\" colspan=\"2\" data-mce-style=\"border: 1px solid black; width: 53.3911%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS_Heat_Inactivated_S101-01_GHS_MSDS.pdf?v=1682397536\" target=\"_blank\" rel=\"noopener noreferrer\"\u003e\u003cstrong\u003eMSDS (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProper storage and thawing method of FBS\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003col data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAfter FBS thawing, why does clots of fleece shape appear? And how affect it to cell culture?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDuring FBS thawing, the most important thing is to shake slowly every 5~10 minutes until thawed all, minimizing bubbles. If the process does not work correctly, concentration gradient with insoluble crystals of fleece shape(cryoprecipitates) can be generated result from imbalance of salt and protein.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe cryoprecipitates is little effect on cell culture. In particular, thawed FBS should not be refrigerated and if you don’t follow the precautions, the clots can be caused a lot of further. Because of this, aliquots of thawing must be frozen keep. The cryoprecipitates is not a defect or fault of product.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003col data-mce-fragment=\"1\" start=\"2\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eWhat is proper storage and use method of FBS?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e1) Proper FBS thawing process(for minimizing deposits)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eWhile FBS thawing, shake it slowly every 5 ~ 10  minutes minimizing bubbles for breaking concentration gradient.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFill water in water bath almost to top of FBS(except cap) and raise temperature of water both by 37\u003c\/span\u003e℃\u003cspan data-mce-fragment=\"1\"\u003e..\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003ePut frozen FBS in water bath and thaw it by shaking slowly ever 5~10 minutes until breaking concentration gradient.  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eAfter thawing all, take out and spray 70% alcohol on the surface of bottle.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eDivide FBS into 20~100ml in clean bench.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFreeze divided FBS as soon as possible. And keep FBS in refrigerator if it is used in a day or two. But keep in mind FBS kept in refrigerator can generate more clots than one in freezer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e If Heat-Inactivation process is needed, proceed as follows (in case of buying products that Heat-Inactivation completed, this process is unnecessary)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e   - Put thawed FBS in 56℃\u003cspan data-mce-fragment=\"1\"\u003e water bath and proceed Heat-Inactivation for 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e  - Shake FBS slowly every 5~10 minutes minimizing bubbles and not making concentration gradient like thawing process. If this Heat-inactivation proceeded irregularly, lots of clots can be generated than thawing process.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e2) Must storage FBS in a freezer without automatic frost-free function. (Avoid storage in a home refrigerator\/freezer, which has frost-free function.)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cu data-mce-fragment=\"1\"\u003eMust Storage in a freezer without automatic frost-free function for preventing deterioration of FBS from temperature change.\u003cspan\u003e \u003c\/span\u003e\u003c\/u\u003e A freezer with automatic frost-free function change the temperature automatically for preventing frost, so it can cause reduce the quality of FBS by freezing and thawing the surface of product repeatedly. And give attention that product bottle can be broken in temperature.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e3) Minimize freezing-thawing process\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTemperature change from freezing-thawing process destroys protein, growth factor, hormone and other nutrient. Thus,\u003cspan\u003e \u003c\/span\u003e\u003cu data-mce-fragment=\"1\"\u003eit is necessary to minimize freezing-thawing process.\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003eAfter first thawing, divide FBS into amount used to once or twice(20~100ml) and frozen. This can reduce iterative freezing-thawing process and thawing hours. At this time attention not to exceed maximum volume 40ml in 50ml conical tube and 90ml in 100ml glass bottle considering of FBS expands as it freezes.\u003c\/p\u003e","brand":"Welgene","offers":[{"title":"100ML","offer_id":43118932590809,"sku":"","price":175000.0,"currency_code":"KRW","in_stock":true},{"title":"500ML","offer_id":43118932558041,"sku":"","price":665000.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/products\/S101_01.jpg?v=1665471417"},{"product_id":"fetal-bovine-serum-fbs-heat-inactivated-s101-04","title":"FBS Stem Cell-Qualified Heat-Inactivated (S101-04)","description":"\u003ctable style=\"border-collapse: collapse;\" width=\"100%\" data-mce-style=\"border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid black; width: 46.6089%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 46.6089%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS.pdf?v=1682397400\" target=\"_blank\"\u003e\u003cstrong\u003e제품 설명서 (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid black; width: 53.3911%; text-align: center;\" colspan=\"2\" data-mce-style=\"border: 1px solid black; width: 53.3911%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS_Heat_Inactivated_S101-04_GHS_MSDS.pdf?v=1682397535\" target=\"_blank\" rel=\"noopener noreferrer\"\u003e\u003cstrong\u003eMSDS (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/STEM1_2048x2048.jpg?v=1667465943\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/STEM2_2048x2048.jpg?v=1667465949\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProper storage and thawing method of FBS\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003col data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAfter FBS thawing, why does clots of fleece shape appear? And how affect it to cell culture?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDuring FBS thawing, the most important thing is to shake slowly every 5~10 minutes until thawed all, minimizing bubbles. If the process does not work correctly, concentration gradient with insoluble crystals of fleece shape(cryoprecipitates) can be generated result from imbalance of salt and protein.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe cryoprecipitates is little effect on cell culture. In particular, thawed FBS should not be refrigerated and if you don’t follow the precautions, the clots can be caused a lot of further. Because of this, aliquots of thawing must be frozen keep. The cryoprecipitates is not a defect or fault of product.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003col data-mce-fragment=\"1\" start=\"2\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eWhat is proper storage and use method of FBS?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e1) Proper FBS thawing process(for minimizing deposits)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eWhile FBS thawing, shake it slowly every 5 ~ 10  minutes minimizing bubbles for breaking concentration gradient.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFill water in water bath almost to top of FBS(except cap) and raise temperature of water both by 37\u003c\/span\u003e℃\u003cspan data-mce-fragment=\"1\"\u003e..\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003ePut frozen FBS in water bath and thaw it by shaking slowly ever 5~10 minutes until breaking concentration gradient.  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eAfter thawing all, take out and spray 70% alcohol on the surface of bottle.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eDivide FBS into 20~100ml in clean bench.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFreeze divided FBS as soon as possible. And keep FBS in refrigerator if it is used in a day or two. But keep in mind FBS kept in refrigerator can generate more clots than one in freezer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e If Heat-Inactivation process is needed, proceed as follows (in case of buying products that Heat-Inactivation completed, this process is unnecessary)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e   - Put thawed FBS in 56℃\u003cspan data-mce-fragment=\"1\"\u003e water bath and proceed Heat-Inactivation for 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e  - Shake FBS slowly every 5~10 minutes minimizing bubbles and not making concentration gradient like thawing process. If this Heat-inactivation proceeded irregularly, lots of clots can be generated than thawing process.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e2) Must storage FBS in a freezer without automatic frost-free function. (Avoid storage in a home refrigerator\/freezer, which has frost-free function.)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cu data-mce-fragment=\"1\"\u003eMust Storage in a freezer without automatic frost-free function for preventing deterioration of FBS from temperature change.\u003cspan\u003e \u003c\/span\u003e\u003c\/u\u003e A freezer with automatic frost-free function change the temperature automatically for preventing frost, so it can cause reduce the quality of FBS by freezing and thawing the surface of product repeatedly. And give attention that product bottle can be broken in temperature.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e3) Minimize freezing-thawing process\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTemperature change from freezing-thawing process destroys protein, growth factor, hormone and other nutrient. Thus,\u003cspan\u003e \u003c\/span\u003e\u003cu data-mce-fragment=\"1\"\u003eit is necessary to minimize freezing-thawing process.\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003eAfter first thawing, divide FBS into amount used to once or twice(20~100ml) and frozen. This can reduce iterative freezing-thawing process and thawing hours. At this time attention not to exceed maximum volume 40ml in 50ml conical tube and 90ml in 100ml glass bottle considering of FBS expands as it freezes.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Welgene","offers":[{"title":"100ML","offer_id":43118934393049,"sku":null,"price":210000.0,"currency_code":"KRW","in_stock":true},{"title":"500ML","offer_id":43531053105369,"sku":"","price":810000.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/products\/S101-04.jpg?v=1672119012"},{"product_id":"fetal-bovine-serum-fbs-heat-inactivated-s101-05-aa","title":"FBS Dialyzed Heat-Inactivated (S101-05)","description":"\u003ctable style=\"border-collapse: collapse;\" width=\"100%\" data-mce-style=\"border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid black; width: 46.6089%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 46.6089%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS.pdf?v=1682397400\" target=\"_blank\"\u003e\u003cstrong\u003e제품 설명서 (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid black; width: 53.3911%; text-align: center;\" colspan=\"2\" data-mce-style=\"border: 1px solid black; width: 53.3911%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS_Heat_Inactivated_S101-05_GHS_MSDS.pdf?v=1682397535\" target=\"_blank\" rel=\"noopener noreferrer\"\u003e\u003cstrong\u003eMSDS (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProper storage and thawing method of FBS\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003col data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAfter FBS thawing, why does clots of fleece shape appear? And how affect it to cell culture?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDuring FBS thawing, the most important thing is to shake slowly every 5~10 minutes until thawed all, minimizing bubbles. If the process does not work correctly, concentration gradient with insoluble crystals of fleece shape(cryoprecipitates) can be generated result from imbalance of salt and protein.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe cryoprecipitates is little effect on cell culture. In particular, thawed FBS should not be refrigerated and if you don’t follow the precautions, the clots can be caused a lot of further. Because of this, aliquots of thawing must be frozen keep. The cryoprecipitates is not a defect or fault of product.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003col data-mce-fragment=\"1\" start=\"2\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eWhat is proper storage and use method of FBS?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e1) Proper FBS thawing process(for minimizing deposits)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eWhile FBS thawing, shake it slowly every 5 ~ 10  minutes minimizing bubbles for breaking concentration gradient.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFill water in water bath almost to top of FBS(except cap) and raise temperature of water both by 37\u003c\/span\u003e℃\u003cspan data-mce-fragment=\"1\"\u003e..\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003ePut frozen FBS in water bath and thaw it by shaking slowly ever 5~10 minutes until breaking concentration gradient.  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eAfter thawing all, take out and spray 70% alcohol on the surface of bottle.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eDivide FBS into 20~100ml in clean bench.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFreeze divided FBS as soon as possible. And keep FBS in refrigerator if it is used in a day or two. But keep in mind FBS kept in refrigerator can generate more clots than one in freezer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e If Heat-Inactivation process is needed, proceed as follows (in case of buying products that Heat-Inactivation completed, this process is unnecessary)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e   - Put thawed FBS in 56℃\u003cspan data-mce-fragment=\"1\"\u003e water bath and proceed Heat-Inactivation for 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e  - Shake FBS slowly every 5~10 minutes minimizing bubbles and not making concentration gradient like thawing process. If this Heat-inactivation proceeded irregularly, lots of clots can be generated than thawing process.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e2) Must storage FBS in a freezer without automatic frost-free function. (Avoid storage in a home refrigerator\/freezer, which has frost-free function.)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cu data-mce-fragment=\"1\"\u003eMust Storage in a freezer without automatic frost-free function for preventing deterioration of FBS from temperature change.\u003cspan\u003e \u003c\/span\u003e\u003c\/u\u003e A freezer with automatic frost-free function change the temperature automatically for preventing frost, so it can cause reduce the quality of FBS by freezing and thawing the surface of product repeatedly. And give attention that product bottle can be broken in temperature.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e3) Minimize freezing-thawing process\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTemperature change from freezing-thawing process destroys protein, growth factor, hormone and other nutrient. Thus,\u003cspan\u003e \u003c\/span\u003e\u003cu data-mce-fragment=\"1\"\u003eit is necessary to minimize freezing-thawing process.\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003eAfter first thawing, divide FBS into amount used to once or twice(20~100ml) and frozen. This can reduce iterative freezing-thawing process and thawing hours. At this time attention not to exceed maximum volume 40ml in 50ml conical tube and 90ml in 100ml glass bottle considering of FBS expands as it freezes.\u003c\/p\u003e","brand":"Welgene","offers":[{"title":"500ML","offer_id":43118945042649,"sku":"","price":840000.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/products\/S101-05.jpg?v=1672119032"},{"product_id":"fetal-bovine-serum-fbs-heat-inactivated-s101-07-aa","title":"FBS Certified Heat-Inactivated (S101-07)","description":"\u003ctable style=\"border-collapse: collapse;\" width=\"100%\" data-mce-style=\"border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid black; width: 46.6089%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 46.6089%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS.pdf?v=1682397400\" target=\"_blank\"\u003e\u003cstrong\u003e제품 설명서 (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid black; width: 53.3911%; text-align: center;\" colspan=\"2\" data-mce-style=\"border: 1px solid black; width: 53.3911%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS_Heat_Inactivated_S101-07_GHS_MSDS.pdf?v=1682397535\" target=\"_blank\" rel=\"noopener noreferrer\"\u003e\u003cstrong\u003eMSDS (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProper storage and thawing method of FBS\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003col data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAfter FBS thawing, why does clots of fleece shape appear? And how affect it to cell culture?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDuring FBS thawing, the most important thing is to shake slowly every 5~10 minutes until thawed all, minimizing bubbles. If the process does not work correctly, concentration gradient with insoluble crystals of fleece shape(cryoprecipitates) can be generated result from imbalance of salt and protein.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe cryoprecipitates is little effect on cell culture. In particular, thawed FBS should not be refrigerated and if you don’t follow the precautions, the clots can be caused a lot of further. Because of this, aliquots of thawing must be frozen keep. The cryoprecipitates is not a defect or fault of product.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003col start=\"2\" data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eWhat is proper storage and use method of FBS?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e1) Proper FBS thawing process(for minimizing deposits)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eWhile FBS thawing, shake it slowly every 5 ~ 10  minutes minimizing bubbles for breaking concentration gradient.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFill water in water bath almost to top of FBS(except cap) and raise temperature of water both by 37\u003c\/span\u003e℃\u003cspan data-mce-fragment=\"1\"\u003e..\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003ePut frozen FBS in water bath and thaw it by shaking slowly ever 5~10 minutes until breaking concentration gradient.  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eAfter thawing all, take out and spray 70% alcohol on the surface of bottle.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eDivide FBS into 20~100ml in clean bench.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFreeze divided FBS as soon as possible. And keep FBS in refrigerator if it is used in a day or two. But keep in mind FBS kept in refrigerator can generate more clots than one in freezer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e If Heat-Inactivation process is needed, proceed as follows (in case of buying products that Heat-Inactivation completed, this process is unnecessary)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e   - Put thawed FBS in 56℃\u003cspan data-mce-fragment=\"1\"\u003e water bath and proceed Heat-Inactivation for 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e  - Shake FBS slowly every 5~10 minutes minimizing bubbles and not making concentration gradient like thawing process. If this Heat-inactivation proceeded irregularly, lots of clots can be generated than thawing process.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e2) Must storage FBS in a freezer without automatic frost-free function. (Avoid storage in a home refrigerator\/freezer, which has frost-free function.)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cu data-mce-fragment=\"1\"\u003eMust Storage in a freezer without automatic frost-free function for preventing deterioration of FBS from temperature change.\u003cspan\u003e \u003c\/span\u003e\u003c\/u\u003e A freezer with automatic frost-free function change the temperature automatically for preventing frost, so it can cause reduce the quality of FBS by freezing and thawing the surface of product repeatedly. And give attention that product bottle can be broken in temperature.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e3) Minimize freezing-thawing process\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTemperature change from freezing-thawing process destroys protein, growth factor, hormone and other nutrient. Thus,\u003cspan\u003e \u003c\/span\u003e\u003cu data-mce-fragment=\"1\"\u003eit is necessary to minimize freezing-thawing process.\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003eAfter first thawing, divide FBS into amount used to once or twice(20~100ml) and frozen. This can reduce iterative freezing-thawing process and thawing hours. At this time attention not to exceed maximum volume 40ml in 50ml conical tube and 90ml in 100ml glass bottle considering of FBS expands as it freezes.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e","brand":"Welgene","offers":[{"title":"500ML","offer_id":43118954545369,"sku":null,"price":635000.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/products\/S101-07.jpg?v=1672119052"},{"product_id":"fetal-bovine-serum-fbs-heat-inactivated-s101-09","title":"FBS, IgG Depleted Heat-Inactivated (S101-09)","description":"\u003ctable style=\"border-collapse: collapse;\" width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid black; width: 46.6089%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS.pdf?v=1682397400\" target=\"_blank\"\u003e\u003cstrong\u003e제품 설명서 (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid black; width: 53.3911%; text-align: center;\" colspan=\"2\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS_Heat_Inactivated_S101-09_GHS_MSDS.pdf?v=1682397535\" rel=\"noopener noreferrer\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProper storage and thawing method of FBS\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003col data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAfter FBS thawing, why does clots of fleece shape appear? And how affect it to cell culture?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDuring FBS thawing, the most important thing is to shake slowly every 5~10 minutes until thawed all, minimizing bubbles. If the process does not work correctly, concentration gradient with insoluble crystals of fleece shape(cryoprecipitates) can be generated result from imbalance of salt and protein.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe cryoprecipitates is little effect on cell culture. In particular, thawed FBS should not be refrigerated and if you don’t follow the precautions, the clots can be caused a lot of further. Because of this, aliquots of thawing must be frozen keep. The cryoprecipitates is not a defect or fault of product.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003col data-mce-fragment=\"1\" start=\"2\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eWhat is proper storage and use method of FBS?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e1) Proper FBS thawing process(for minimizing deposits)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eWhile FBS thawing, shake it slowly every 5 ~ 10  minutes minimizing bubbles for breaking concentration gradient.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFill water in water bath almost to top of FBS(except cap) and raise temperature of water both by 37\u003c\/span\u003e℃\u003cspan data-mce-fragment=\"1\"\u003e..\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003ePut frozen FBS in water bath and thaw it by shaking slowly ever 5~10 minutes until breaking concentration gradient.  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eAfter thawing all, take out and spray 70% alcohol on the surface of bottle.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eDivide FBS into 20~100ml in clean bench.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFreeze divided FBS as soon as possible. And keep FBS in refrigerator if it is used in a day or two. But keep in mind FBS kept in refrigerator can generate more clots than one in freezer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e If Heat-Inactivation process is needed, proceed as follows (in case of buying products that Heat-Inactivation completed, this process is unnecessary)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e   - Put thawed FBS in 56℃\u003cspan data-mce-fragment=\"1\"\u003e water bath and proceed Heat-Inactivation for 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e  - Shake FBS slowly every 5~10 minutes minimizing bubbles and not making concentration gradient like thawing process. If this Heat-inactivation proceeded irregularly, lots of clots can be generated than thawing process.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e2) Must storage FBS in a freezer without automatic frost-free function. (Avoid storage in a home refrigerator\/freezer, which has frost-free function.)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cu data-mce-fragment=\"1\"\u003eMust Storage in a freezer without automatic frost-free function for preventing deterioration of FBS from temperature change.\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003c\/u\u003e A freezer with automatic frost-free function change the temperature automatically for preventing frost, so it can cause reduce the quality of FBS by freezing and thawing the surface of product repeatedly. And give attention that product bottle can be broken in temperature.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e3) Minimize freezing-thawing process\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTemperature change from freezing-thawing process destroys protein, growth factor, hormone and other nutrient. Thus,\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cu data-mce-fragment=\"1\"\u003eit is necessary to minimize freezing-thawing process.\u003c\/u\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eAfter first thawing, divide FBS into amount used to once or twice(20~100ml) and frozen. This can reduce iterative freezing-thawing process and thawing hours. At this time attention not to exceed maximum volume 40ml in 50ml conical tube and 90ml in 100ml glass bottle considering of FBS expands as it freezes.\u003c\/p\u003e","brand":"Welgene","offers":[{"title":"100ML - 본사 문의","offer_id":43118961361113,"sku":null,"price":0.0,"currency_code":"KRW","in_stock":true},{"title":"500ML - 본사 문의","offer_id":43118961328345,"sku":null,"price":0.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/S101-09_FBS_IgGDepletedHeat-Inactivated.jpg?v=1739944884"},{"product_id":"fetal-bovine-serum-fbs-dialyzed","title":"FBS, Dialyzed (S001-05)","description":"\u003ctable style=\"border-collapse: collapse;\" width=\"100%\" data-mce-style=\"border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid black; width: 46.6089%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 46.6089%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS.pdf?v=1682397400\" target=\"_blank\"\u003e\u003cstrong\u003e제품 설명서 (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid black; width: 53.3911%; text-align: center;\" colspan=\"2\" data-mce-style=\"border: 1px solid black; width: 53.3911%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS_S001-05_GHS_MSDS.pdf?v=1682391595\" target=\"_blank\" rel=\"noopener noreferrer\"\u003e\u003cstrong\u003eMSDS (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProper storage and thawing method of FBS\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003col data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAfter FBS thawing, why does clots of fleece shape appear? And how affect it to cell culture?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDuring FBS thawing, the most important thing is to shake slowly every 5~10 minutes until thawed all, minimizing bubbles. If the process does not work correctly, concentration gradient with insoluble crystals of fleece shape(cryoprecipitates) can be generated result from imbalance of salt and protein.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe cryoprecipitates is little effect on cell culture. In particular, thawed FBS should not be refrigerated and if you don’t follow the precautions, the clots can be caused a lot of further. Because of this, aliquots of thawing must be frozen keep. The cryoprecipitates is not a defect or fault of product.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003col start=\"2\" data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eWhat is proper storage and use method of FBS?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e1) Proper FBS thawing process(for minimizing deposits)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eWhile FBS thawing, shake it slowly every 5 ~ 10  minutes minimizing bubbles for breaking concentration gradient.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFill water in water bath almost to top of FBS(except cap) and raise temperature of water both by 37\u003c\/span\u003e℃\u003cspan data-mce-fragment=\"1\"\u003e..\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003ePut frozen FBS in water bath and thaw it by shaking slowly ever 5~10 minutes until breaking concentration gradient.  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eAfter thawing all, take out and spray 70% alcohol on the surface of bottle.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eDivide FBS into 20~100ml in clean bench.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFreeze divided FBS as soon as possible. And keep FBS in refrigerator if it is used in a day or two. But keep in mind FBS kept in refrigerator can generate more clots than one in freezer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e If Heat-Inactivation process is needed, proceed as follows (in case of buying products that Heat-Inactivation completed, this process is unnecessary)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e   - Put thawed FBS in 56℃\u003cspan data-mce-fragment=\"1\"\u003e water bath and proceed Heat-Inactivation for 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e  - Shake FBS slowly every 5~10 minutes minimizing bubbles and not making concentration gradient like thawing process. If this Heat-inactivation proceeded irregularly, lots of clots can be generated than thawing process.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e2) Must storage FBS in a freezer without automatic frost-free function. (Avoid storage in a home refrigerator\/freezer, which has frost-free function.)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cu data-mce-fragment=\"1\"\u003eMust Storage in a freezer without automatic frost-free function for preventing deterioration of FBS from temperature change.\u003cspan\u003e \u003c\/span\u003e\u003c\/u\u003e A freezer with automatic frost-free function change the temperature automatically for preventing frost, so it can cause reduce the quality of FBS by freezing and thawing the surface of product repeatedly. And give attention that product bottle can be broken in temperature.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e3) Minimize freezing-thawing process\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTemperature change from freezing-thawing process destroys protein, growth factor, hormone and other nutrient. Thus,\u003cspan\u003e \u003c\/span\u003e\u003cu data-mce-fragment=\"1\"\u003eit is necessary to minimize freezing-thawing process.\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003eAfter first thawing, divide FBS into amount used to once or twice(20~100ml) and frozen. This can reduce iterative freezing-thawing process and thawing hours. At this time attention not to exceed maximum volume 40ml in 50ml conical tube and 90ml in 100ml glass bottle considering of FBS expands as it freezes.\u003c\/p\u003e","brand":"Welgene","offers":[{"title":"100ML","offer_id":43119176384729,"sku":null,"price":210000.0,"currency_code":"KRW","in_stock":true},{"title":"500ML","offer_id":43119176351961,"sku":null,"price":830000.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/products\/S001-05.jpg?v=1672118837"},{"product_id":"igg-depleted","title":"FBS, IgG Depleted (S001-09)","description":"\u003ctable style=\"border-collapse: collapse;\" width=\"100%\" data-mce-style=\"border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid black; width: 46.6089%; text-align: center;\" data-mce-style=\"border: 1px solid black; width: 46.6089%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS.pdf?v=1682397400\" target=\"_blank\"\u003e\u003cstrong\u003e제품 설명서 (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid black; width: 53.3911%; text-align: center;\" colspan=\"2\" data-mce-style=\"border: 1px solid black; width: 53.3911%; text-align: center;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/files\/FBS_S001-09_GHS_MSDS.pdf?v=1682391595\" target=\"_blank\" rel=\"noopener noreferrer\"\u003e\u003cstrong\u003eMSDS (PDF 파일 다운로드)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProper storage and thawing method of FBS\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003col data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAfter FBS thawing, why does clots of fleece shape appear? And how affect it to cell culture?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDuring FBS thawing, the most important thing is to shake slowly every 5~10 minutes until thawed all, minimizing bubbles. If the process does not work correctly, concentration gradient with insoluble crystals of fleece shape(cryoprecipitates) can be generated result from imbalance of salt and protein.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe cryoprecipitates is little effect on cell culture. In particular, thawed FBS should not be refrigerated and if you don’t follow the precautions, the clots can be caused a lot of further. Because of this, aliquots of thawing must be frozen keep. The cryoprecipitates is not a defect or fault of product.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003col start=\"2\" data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eWhat is proper storage and use method of FBS?\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e1) Proper FBS thawing process(for minimizing deposits)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eWhile FBS thawing, shake it slowly every 5 ~ 10  minutes minimizing bubbles for breaking concentration gradient.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFill water in water bath almost to top of FBS(except cap) and raise temperature of water both by 37\u003c\/span\u003e℃\u003cspan data-mce-fragment=\"1\"\u003e..\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003ePut frozen FBS in water bath and thaw it by shaking slowly ever 5~10 minutes until breaking concentration gradient.  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eAfter thawing all, take out and spray 70% alcohol on the surface of bottle.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eDivide FBS into 20~100ml in clean bench.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan\u003e \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eFreeze divided FBS as soon as possible. And keep FBS in refrigerator if it is used in a day or two. But keep in mind FBS kept in refrigerator can generate more clots than one in freezer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e★\u003cspan data-mce-fragment=\"1\"\u003e If Heat-Inactivation process is needed, proceed as follows (in case of buying products that Heat-Inactivation completed, this process is unnecessary)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e   - Put thawed FBS in 56℃\u003cspan data-mce-fragment=\"1\"\u003e water bath and proceed Heat-Inactivation for 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e  - Shake FBS slowly every 5~10 minutes minimizing bubbles and not making concentration gradient like thawing process. If this Heat-inactivation proceeded irregularly, lots of clots can be generated than thawing process.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e2) Must storage FBS in a freezer without automatic frost-free function. (Avoid storage in a home refrigerator\/freezer, which has frost-free function.)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cu data-mce-fragment=\"1\"\u003eMust Storage in a freezer without automatic frost-free function for preventing deterioration of FBS from temperature change.\u003cspan\u003e \u003c\/span\u003e\u003c\/u\u003e A freezer with automatic frost-free function change the temperature automatically for preventing frost, so it can cause reduce the quality of FBS by freezing and thawing the surface of product repeatedly. And give attention that product bottle can be broken in temperature.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e3) Minimize freezing-thawing process\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTemperature change from freezing-thawing process destroys protein, growth factor, hormone and other nutrient. Thus,\u003cspan\u003e \u003c\/span\u003e\u003cu data-mce-fragment=\"1\"\u003eit is necessary to minimize freezing-thawing process.\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003eAfter first thawing, divide FBS into amount used to once or twice(20~100ml) and frozen. This can reduce iterative freezing-thawing process and thawing hours. At this time attention not to exceed maximum volume 40ml in 50ml conical tube and 90ml in 100ml glass bottle considering of FBS expands as it freezes.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e","brand":"Welgene","offers":[{"title":"500ML","offer_id":43119180775641,"sku":null,"price":850000.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0632\/0367\/2281\/products\/S001-09.jpg?v=1672118890"}],"url":"https:\/\/en.welgene.com\/ko\/collections\/fetal-bovine-sera-fbs-1.oembed","provider":"Welgene","version":"1.0","type":"link"}